Composite
Part:BBa_K3028001:Design
Designed by: Yulia Kacher Group: iGEM19_Moscow (2019-10-14)
С-terminal part of beta-lactamase fused with dCas9 and double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1150
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3429 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was necessary to make such an improvement, because of all side effects of Cas synthesis for cells.
Source
Original Part BBa_K1689014